Isopropylmalate synthase <i>NtIPMS</i> as a potential molecular marker for seed vigor in tobacco

Seed vigor is an important trait for tobacco production. However, the evaluation of seed using molecular biomarkers scarcely reported in tobacco. In this study, development marker isopropylmalate synthase NtIPMS was conducted to detect ageing degree and priming effect Quantitative real-time PCR (qRT-PCR) analysis showed that expression significantly induced at initial imbibition stage during germination. The positively correlated with non-pelleted pelleted seeds. mRNA level gradually increased increasing ageing. early best gibberellin observed 30-h primed seeds, highest 12-h stop time-point likely time 18 h after relatively higher occurred process. detection has potential usage as a